PhD in experimental biophysics, University of Illinois, Chicago, USA (2005)
PhD, Institute of Theoretical Physics of CAS, China (1998)

Office : S7-01-10  |  Tel : (65) 6516 2620
Email : phyyj@nus.edu.sg

Current Research

Our research is based on advanced single-molecule manipulation and imaging technologies. Current research interests include:

  • Molecular mechanisms of mechanosensing of cells
  • Interfering mechanosensing of cells using compounds
  • DNA packaging, gene regulation and DNA damage repair
  • Bacterial biofilm initiation and maintenance

Schematic representation of a magnetic tweezers setup to stretch short DNA molecules or proteins. (A) The DNA or protein is tethered between a paramagnetic bead and a coverslip surface. Force is applied to the bead by a pair of permanent magnets above the sample, and changing the distance between the magnets and the bead controls the magnitude of the force over a range from 0.1 pN to 200 pN. A bead stuck on the surface is used as a reference to eliminate drift in three dimensions. (B) Schematic representation of the application of the instrument to study interaction between a short DNA and a DNA bending protein. Association and disassociation of the protein lead to change in DNA extension which is monitored by the change in the height of the bead at a sub-nanometer resolution in real time.

Selected Publications

  1. Yao, M., Goult, B. T., Klapholz, B., Hu, X., Toseland, C.P., Guo, Y., Cong, P., Sheetz, M. P., and Yan, J. (2016). "The mechanical response of talin." Nature Communications, 7: 11966.

  2. Chen, H., Yuan, G., Winardhi, R.S., Yao, M., Popa, I., Fernandez, J.M., Yan, J. (2015). "Dynamics of equilibrium folding and unfolding transitions of titin immunoglobulin domain under constant forces." JACS, 137: 3540-3546.

  3. You, H., Wu, J., Shao, F., Yan, J. (2015). "Stability and Kinetics of c-MYC Promoter G-Quadruplexes Studied by Single-Molecule Manipulation." JACS, 137: 2424-2427.

  4. Zhang, X., Qu, Y., Chen, H., Rouzina, I., Zhang, S., Doyle, P., Yan, J. (2014). "Interconversion between three overstretched DNA structures." JACS, 136: 16073 -16080.

  5. Yao, M., Qiu, W., Liu, R. , Efremov, A.K., Cong, P., Seddiki, R., Payre, M., Lim, C.T., Ladoux, B., Me`ge, R.M., and Yan, J. (2014). "Force-dependent conformational switch of alpha-catenin controls vinculin binding." Nature Communications, 5:4525.

  6. Zhang, X., Chen, H., Le, S., Rouzina, I., Doyle, P.S., and Yan, J. (2013). "Revealing the Competition between Peeled-ssDNA, Melting Bubbles and S-DNA during DNA Overstretching by Single-Molecule Calorimetry." PNAS, 110:3859-3864.

Affiliations & Links